ptwin1 plasmid (New England Biolabs)
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New England Biolabs
ptwin1 plasmid
Ptwin1 Plasmid, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptwin1+plasmid/pTWIN1+Vector+DNA/pmc03620165-58-32-34
Average 93 stars, based on 218 article reviews
Ptwin1 Plasmid, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptwin1+plasmid/pTWIN1+Vector+DNA/pmc03620165-58-32-34
Average 93 stars, based on 218 article reviews
ptwin1 plasmid - by Bioz Stars,
2026-10
93/100 stars
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Synthesized:Article Title: Expression, Purification, and Characterization of Human Cystatin C Monomers and Oligomers Article Snippet: Cystatin C production and purification The human cystatin C gene was codon-optimized for expression in E. coli using Genscript's OptimumGene algorithm ( Supplementary Figure S1 ). .. The gene was synthesized with NruI and BamHI restriction sites at 5′ and 3′ positions, respectively, and ligated into the Article Title: Retinol Binding Protein IV Purified from Escherichia coli Using Intein-Mediated Cleavage as a Suitable Replacement for Serum Sources Article Snippet: The human RBP IV gene without the signaling peptide (UniProt KB {"type":"entrez-protein","attrs":{"text":"P02753","term_id":"62298174"}} P02753 ) was codon-optimized for expression in E. coli using Genscript’s OptimumGene algorithm. .. The gene was synthesized using the Nrul and BamHI restriction sites at the 5’ and 3’ positions, respectively, and ligated into the Plasmid Preparation:Article Title: Expression, Purification, and Characterization of Human Cystatin C Monomers and Oligomers Article Snippet: Cystatin C production and purification The human cystatin C gene was codon-optimized for expression in E. coli using Genscript's OptimumGene algorithm ( Supplementary Figure S1 ). .. The gene was synthesized with NruI and BamHI restriction sites at 5′ and 3′ positions, respectively, and ligated into the Article Title: Protein Trans -Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins Article Snippet: .. To construct plasmids expressing only the C-protein for S1 split inteins, the C-protein coding sequence was isolated from the split intein plasmid as an Nde I- Pst I fragment and inserted into Article Title: Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System Article Snippet: .. The Ssp DnaB mini-intein gene was amplified from the Article Title: Retinol Binding Protein IV Purified from Escherichia coli Using Intein-Mediated Cleavage as a Suitable Replacement for Serum Sources Article Snippet: The human RBP IV gene without the signaling peptide (UniProt KB {"type":"entrez-protein","attrs":{"text":"P02753","term_id":"62298174"}} P02753 ) was codon-optimized for expression in E. coli using Genscript’s OptimumGene algorithm. .. The gene was synthesized using the Nrul and BamHI restriction sites at the 5’ and 3’ positions, respectively, and ligated into the Article Title: Human RPA activates BLM’s bidirectional DNA unwinding from a nick Article Snippet: Recombinant DNA reagent , pBR322 (plasmid) , Takara , RRID: Addgene_10877 , . .. Recombinant DNA reagent , Article Title: Using self-cleavable ternary fusion pattern for efficient preparation of Bacteriorhodopsin Article Snippet: The study of membrane proteins has been notoriously hampered by its low expression level and difficulty in purification and crystallization.. Therefore, development of efficient method for preparation of properly folded membrane proteins with milligram yield would be of critical importance.. Here, we developed a simple and efficient strategy to obtain high purity of bacterio-opsion (bO), the apoprotein of bacteriorhodopsin (bR), in Escherichia coli (E. coli) using self-cleavable ternary fusion pattern. Construct:Article Title: Protein Trans -Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins Article Snippet: .. To construct plasmids expressing only the C-protein for S1 split inteins, the C-protein coding sequence was isolated from the split intein plasmid as an Nde I- Pst I fragment and inserted into Expressing:Article Title: Protein Trans -Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins Article Snippet: .. To construct plasmids expressing only the C-protein for S1 split inteins, the C-protein coding sequence was isolated from the split intein plasmid as an Nde I- Pst I fragment and inserted into Article Title: Human RPA activates BLM’s bidirectional DNA unwinding from a nick Article Snippet: Recombinant DNA reagent , pBR322 (plasmid) , Takara , RRID: Addgene_10877 , . .. Recombinant DNA reagent , Sequencing:Article Title: Protein Trans -Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins Article Snippet: .. To construct plasmids expressing only the C-protein for S1 split inteins, the C-protein coding sequence was isolated from the split intein plasmid as an Nde I- Pst I fragment and inserted into Isolation:Article Title: Protein Trans -Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins Article Snippet: .. To construct plasmids expressing only the C-protein for S1 split inteins, the C-protein coding sequence was isolated from the split intein plasmid as an Nde I- Pst I fragment and inserted into Amplification:Article Title: Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System Article Snippet: .. The Ssp DnaB mini-intein gene was amplified from the Recombinant:Article Title: Human RPA activates BLM’s bidirectional DNA unwinding from a nick Article Snippet: Recombinant DNA reagent , pBR322 (plasmid) , Takara , RRID: Addgene_10877 , . .. Recombinant DNA reagent , |